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KMID : 0545120140240010036
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Journal of Microbiology and Biotechnology
2014 Volume.24 No. 1 p.36 ~ p.43
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Peroxidase and Photoprotective Activities of Magnesium Protoporphyrin IX
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Kim Eui-Jin
Oh Eun-Kyoung
Lee Jeong-K.
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Abstract
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Magnesium-protoporphyrin IX (Mg-PPn), which is formed through chelation of protoporphyrin IX (PPn) with Mg ion by Mg chelatase, is the first intermediate for the (bacterio)chlorophyll biosynthetic pathway. Interestingly, Mg-PPn provides peroxidase activity (approximately 4 × 10-2 units/μM) detoxifying H2O2 in the presence of electron donor(s). The peroxidase activity was not detected unless PPn was chelated with Mg ion. Mg-PPn was found freely diffusible through the membrane of Escherichia coli and Vibrio vulnificus, protecting the cells from H2O2. Furthermore, unlike photosensitizers such as tetracycline and PPn, Mg-PPn did not show any phototoxicity, but rather it protected cell from ultraviolet (UV)-A-induced stress. Thus, the exogenous Mg-PPn could be used as an antioxidant and a UV block to protect cells from H2O2 stress and UV-induced damage.
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KEYWORD
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Mg-protoporphyrin IX, tetrapyrrole, metallo-porphyrin, peroxidase, photo-protectant
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